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Journal of Gerontology 1984 39(1):11-17; doi:10.1093/geronj/39.1.11
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© 1984 The Gerontological Society of America

Progressive Loss of the Proliferative Response of Senescing Wi-38 Cells to Platelet-derived Growth Factor, Epidermal Growth Factor, Insulin, Transferrin, and Dexamethasone1

Paul D. Phillips, PhD2, Kazuhiko Kaji, PhD3 and Vincent J. Cristofalo, PhD2

2 The Wistar Institute of Anatomy and Biology 36th Street at Spruce, Philadelphia, PA 19104.


  Abstract

Normal human diploid cell lines such as WI-38 display a progressive loss of responsiveness to the mitogenic components in serum. Using a serum-free, hormone-growth factor supplemented medium for WI-38 cells (Phillips & Cristofalo, 1980,1981), we examined the dose-response relationships of cells to the individual growth factor at various in vitro ages. Although as cultures senesced, they became progressively less responsive to mitogenic stimulation, the concentration of epidermal growth factor (25 ng/ml), insulin (5 /µg/ml), transferrin (5 /µg/ml), and dexamethasone (55 ng/ml) that elicited the maximum proliferative response did not change as a function of age. As cultures age they may require increasing amounts of platelet-derived growth factor to elicit the maximum mitogenic response.

Key Words: Cellular senescence • Growth factors • Hormones

1 This work was supported by grants AG-00378 and AG-02851 from the National Institutes of Health. Address all correspondence to Vincent J. Cristofalo, PhD. The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, PA 19104.

3 Present address: 1 Tokyo Metropolitan Institute of Gerontology, Tokyo—173, Japan.


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